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KMID : 0613820070170010143
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Journal of Life Science
2007 Volume.17 No. 1 p.143 ~ p.149
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Inhibition effect of neuronal death by Scutellaria baicalensis GEORGI Water-Extract in a Hypoxic Model of Cultured Rat Cortical Cells
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Kim Wan-Sikd
Jeong Seung-Hyun
Shin Gil-Cho
Moon Ill-Soo
Lee Won-Chul
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Abstract
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Scutellaria baicalensis GEORGI(SB) is used in oriental medicine for the treatment of incipient strokes. Although it has been reported that SB is neuroprotective in a hypoxiamodel, its mechanism is poorly understood. Here, we investigated the effect of SB on the modulation of retinoic acid receptor α (RARα). Rat cerebrocortical cells were grown in neurobasal medium. On DIV12 cells were treated with SB (20 ㎍/㎖) and given a hypoxic shock (2％ O₂／5％ CO2, 3 hr) on DIV14. In situ hybridization using cRNA probe revealed that RARα mRNA punctae are distributed, in addition to nucleus, throughout neuronal dendrites, where SB upregulated its density by 69.8% (p=0.001) and 129.8% (p=0.001) in both normoxia and hypoxia, respectively. At the protein level, SB upregulated RARα in the neuronal soma by 78.8% (p=0.004) and 23.6% (p=0.001) in both normoxia and hypoxia, respectively. These results indicate that SB upregulates RARα in both normoxia and hypoxia, which might contribute to the neuroprotection.
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KEYWORD
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rat cortical cell culture, hypoxia, IC, ISH, RARα, Scutellaria baicalensis GEORGI
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